Table 3.
Sensitivity, specificity, and predictive values of the PCR/ESI-MS Mycobacterium assay for identification and species identification of 264 mycobacterial isolates directly from AFB-positive MGIT broth culture mediaa
| Mycobacterial species, complex, or group, according to PCR/ESI-MS | No. of isolates with indicated PCR/ESI-MS result |
Sensitivity (%) | Specificity (%) | PPV (%) | NPV (%) | |||
|---|---|---|---|---|---|---|---|---|
| Correct positive | Incorrect positive | Incorrect negative | Correct negative | |||||
| M. tuberculosis complex | 49 | 1 | 0 | 218 | 98.0 | 100.0 | 100.0 | 99.5 |
| M. avium/M. intracellulare | 157 | 0 | 1 | 106 | 100.0 | 99.1 | 99.4 | 100.0 |
| M. chelonae/M. abscessus group | 15 | 2 | 0 | 249 | 88.2 | 100.0 | 100.0 | 99.2 |
| M. fortuitum group | 7 | 0 | 0 | 259 | 100.0 | 100.0 | 100.0 | 100.0 |
| M. gordonae | 10 | 2 | 0 | 256 | 83.3 | 100.0 | 100.0 | 99.2 |
| M. kansasii | 16 | 3 | 0 | 249 | 84.2 | 100.0 | 100.0 | 98.8 |
| M. simiae | 1 | 0 | 0 | 267 | 100.0 | 100.0 | 100.0 | 100.0 |
| M. szulgai | 3 | 0 | 0 | 265 | 100.0 | 100.0 | 100.0 | 100.0 |
| Total | 258 | 8 | 1 | 1869 | 97.0 | 99.9 | 99.6 | 99.6 |
PPV, positive predictive value; NPV, negative predictive value.