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. 2010 Dec 22;85(5):2126–2137. doi: 10.1128/JVI.01531-10

FIG. 3.

FIG. 3.

IFITM proteins inhibit an early step of HIV-1 replication. (A) IFITM2 and IFITM3 diminished the number of HIV-1-infected cells. Following doxycycline treatment (0 and 500 ng/ml) for 16 h, SupT1 cells were infected with the NLEY1-IRES virus, which expresses YFP. Forty hours after infection, the number of YFP-positive cells was scored by flow cytometry. (B) IFITM proteins suppressed production of infectious HIV-1 particles. The amounts of infectious virus particles were determined by infecting TZM-bl indicator cells. The fold change was calculated by dividing the values from doxycycline-treated cells (+Dox) by the values from untreated cells (−Dox). The results shown are the averages of three independent infection experiments shown in panel A. The error bars indicate standard deviations. (C) SupT1 cells were infected with the NLEY1-IRES virus for 2 h before exposure to doxycycline (500 ng/ml) treatment. The effects of IFITM proteins on the number of infected cells and the amounts of infectious HIV-1 are summarized in the bar graph. (D) The IFITM SupT1 cell lines were first treated with Dox (500 ng/ml) for 16 h and then infected with NLEY1-ES-IRES viruses that were pseudotyped with VSV G protein. The number of YFP-positive cells was scored 40 h after infection. The results of three independent infections are summarized in the bar graph.