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Association of IFITM2 and IFITM3 with endocytosis. 293 cells were transiently transfected with vector DNA that expressed Flag-tagged IFITM1, IFITM2, or IFITM3. Forty hours after transfection, the cells were fed with Alexa Fluor 555-conjugated transferrin (red) at 37°C for 10 min before being fixed with 4% paraformaldehyde (in 1× phosphate-buffered saline). IFITM proteins (green) were detected by immunostaining them with anti-Flag antibody.
