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. 2010 Dec 22;85(5):2126–2137. doi: 10.1128/JVI.01531-10

FIG. 7.

FIG. 7.

The anti-HIV-1 activity of IFITM1 does not depend on its C-terminal sequence. (A) Illustration of IFITM1 deletion mutants. Sequences from the IFITM1 C terminus were deleted. (B) Doxycycline-induced expression of IFITM1 mutants in SupT1 cells. (C) HIV-1 replication in SupT1 cells that express IFITM1 mutants. SupT1 cells were cultured in media with or without doxycycline (500 ng/ml). The results shown represent three independent spreading-infection experiments. (D) Infection of SupT1 cells with the NLEY1-IRES virus. For details, refer to the legend to Fig. 3B. (E) Comparison of doxycycline-induced expression of IFITM1 and Δ(108-125) to that of IFN-α2b-induced endogenous IFITM1. Cell lines were exposed to doxycycline (500 ng/ml), IFN-α2b (500 U/ml), or both for 16 h before the levels of IFITM1 and Δ(108-125) were assessed in Western blots using anti-IFITM1 antibody. Tubulin was probed as the internal control.