FIG. 2.

Importance of Sp1/Sp3 binding site on ZID for PKCδ induction of Zp. (A) Schematic diagram of defined domain structure and transcription factor binding sites on Zp (−221 to +12). The sequence mutation of ZID reporter mutants is also indicated at the bottom. (B) Analysis of luciferase activities of ZID reporter mutants upon PKCδ induction. NPC-TW01 cells were cotransfected with ZID mutants, −221 Zp, ΔZID, or vector control (pGL2) in combination with EGFP-derived PKCδ expression plasmids (EGFP, EGFP-WT-PKCδ, or EGFP-CF-PKCδ) and Renilla luciferase plasmid (pRL-TK). After 48 h of transfection, the luciferase and Renilla activities were measured and calculated by normalizing the luciferase activity to the Renilla activity and then standardized with that of control vector EGFP. Each experiment was carried out in duplicate and error bars are shown.