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. 2010 Dec 15;85(5):2386–2396. doi: 10.1128/JVI.01855-10

FIG. 1.

FIG. 1.

ERp57, ERp72, and PDI facilitate Py infection. (A) Knockdown of PDI family members. NIH 3T3 cells were transfected with the indicated siRNA, and lysates derived from the cells were subjected to SDS-PAGE and immunoblotted with the indicated antibodies. (B) Induction of XBP1 splicing. Shown is RT-PCR analysis of the unspliced (u) and spliced (s) forms of the XBP1 mRNA from cells treated with DTT or tunicamycin or transfected with the indicated siRNA. (C) Py infection in knockdown cells. Cells transfected with scrambled siRNA or one of two independent siRNAs against ERp57, ERp72, and PDI (siRNAs 1 and 2) were challenged with Py (100 PFU/cell), and the large T antigen expression was analyzed by standard immunofluorescence microscopy. The values were normalized to scrambled siRNA. The data represent the means and standard deviations (SD) of at least three independent experiments. (D) As for panel A, except the indicated siRNAs were used. (E) As for panel B, except the indicated siRNAs were used. (F) As for panel C, except the indicated siRNAs were used. (G) Average infection of single- and double-knockdown cells. A two-tailed t test was used.