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. 2010 Dec 22;85(5):2079–2088. doi: 10.1128/JVI.02279-10

FIG. 6.

FIG. 6.

EBNA3C blocks ING4- and ING5-triggered p53 transcriptional activities. (A to I) Saos-2 (p53−/−) cells were cotransfected with 2 μg of a wild-type p21WAF1/CIP1 promoter construct and different combinations of plasmids expressing untagged EBNA3C, myc-p53, and either flag-tagged ING4 or flag-tagged ING5 as described in Materials and Methods. At 36 h posttransfection, cells were harvested and lysed in reporter lysis buffer. Total amounts of proteins were normalized by the Bradford assay. The bars plot the means of the results of two independent experiments. Error bars represent standard deviations (SD). Different numbers of asterisks represent identical bars in panels A to E. A single asterisk represents the p21WAF1/CIP1 promoter plus the vector control in panels A to I; two asterisks represent the p21WAF1/CIP1 promoter plus 1 μg of a myc-tagged p53-expressing vector in panels A to D and G; three asterisks represent the p21WAF1/CIP1 promoter plus 20 μg of a flag-tagged ING4-expressing vector in panels C, E, and H; and four asterisks represent the p21WAF1/CIP1 promoter plus 20 μg of a flag-tagged ING5-expressing vector in panels D, F, and I.