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. 2010 Dec 15;85(5):2288–2295. doi: 10.1128/JVI.01961-10

FIG. 5.

FIG. 5.

MIR2 is palmitoylated in vivo. Cells infected with KSHV were used to determine the palmitoylation status of MIR2. Cellular lysates from latently infected TREx-BCBL1-Rta cells or TREx-BCBL1-Rta cells that were lytically reactivated were subjected to the biotin switch assay where protein palmitoylation is replaced with biotinylation as described for Fig. 3A. The presence of biotinylated MIR2 was detected by probing Western blots for MIR2. MIR2 was also immunoprecipitated from cellular lysates using an anti-MIR2 antibody to confirm the presence of MIR2 in reactivated cellular lysates.