Coverage of the reference genome obtained with filtered, trimmed reads. (A) First data set (run), (B) second data set (run). The three samples (inoculum, front left foot, and back right foot) received generous coverage from both runs, while fluctuations were higher for the first data set. Average coverage values were ×4,873 (inoculum), ×8,665 (FLF sample), and ×6,594 (BRF sample) for the first data set and ×16,827 (inoculum), ×11,924 (FLF sample), and ×15,945 (BRF sample) for the second data set. At the top of the figure, the sequenced fraction of the genome (nt 368 to 8176) is represented, together with the position of the polyprotein.