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Expression of the TAK1 mutant reduces vGPCR-induced NF-κB target genes. HEK293T cells were transfected with 1 μg of vGPCR and/or 1 μg of TAK1M expression plasmids. Real-time PCR analysis measured the level of message for the indicated genes. (A) Gro1. (B) IL-8. (C) ΙκΒ-α. (D) COX-2. (E) cIAP2. (F) Bcl2. Each reaction was done in triplicate, and means and SD are shown. **, P < 0.01 comparing the vGPCR-plus-TAK1M condition with the vGPCR-alone condition.
