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Phosphorylation of Chk1 following adenovirus infection. HeLa cells were infected with Ad3, Ad4, Ad5, Ad7, Ad9, Ad11, and Ad12, harvested at the times shown, and Western blotted for phospho-Chk1 (Ser345) (A) and Chk1 (B). The final track in the Ad12 phospho-Chk1 blots shows HeLa cell extracts treated with hydroxyurea (HU) as a positive control.
