TABLE 2.
Sensitivity of R5 envelopes and their corresponding mutants with V1 loop residue 153 substitutions to various entry inhibitors and neutralizing antibodies
| Envelope | IC50 |
||||||
|---|---|---|---|---|---|---|---|
| 17b (μg/ml) | 2G12 (μg/ml) | Maraviroc (nM) | 2F5 (μg/ml) | T20 (nM) | PG9 (μg/ml) | PG16 (μg/ml) | |
| 98-15 | >50 | 1.05 | 5.89 | 4 | 40.59 | 0.056 | 0.0049 |
| 98-15 G153E | >50 | 1.16 | 10.2 | 3 | 65.48 | 0.02 | 0.007 |
| 98-27 | >50 | 2.55 | 12.9 | 7.41 | 58.86 | 0.05 | 0.02 |
| 98-27 E153G | >50 | 1.5 | 10.0 | 1.78 | 61.62 | 0.09 | 0.02 |
| 98-28 | >50 | 0.88 | 1.72 | 2.03 | 74.89 | <0.006 | <0.006 |
| 98-28 E153G | >50 | 1.08 | 0.92 | 3.60 | 33.43 | 0.01 | <0.006 |
| JRCSF | >50 | 1.5 | 5.12 | 7.11 | 16.62 | <0.006 | <0.006 |
| JRCSF E153G | >50 | 1.1 | 3.99 | 6.33 | 28.54 | 0.02 | <0.006 |
| LN27 | >50 | 0.59 | 3.66 | 8.2 | 32.87 | >1.56 | >1.56 |
| LN27 E153G | >50 | 0.17 | 0.72 | 5.5 | 28.41 | >1.56 | >1.56 |
| LN8 | 24 | 3.15 | 2.0 | 6.34 | 208.2 | 0.06 | 0.04 |
| LN8 E153G | 24 | 3.77 | 2.82 | 8.09 | 278.1 | >1.56 | >1.56 |
| LN40 | >50 | 1.97 | 2.01 | 2.33 | 42.37 | >1.56 | >1.56 |
| LN40 E153G | >50 | 3.53 | 2.28 | 0.84 | 97.00 | >1.56 | >1.56 |
| FL5-2-209 | >50 | >50 | 0.47 | >50 | 115.50 | >1.56 | >1.56 |
| FL5-2-209 G153E | 37.5 | >50 | 0.38 | >50 | 131.20 | >1.56 | 0.22 |
| 25925-2 cl.22 | 20 | >50 | 0.29 | >50 | 70.00 | 0.04 | 0.01 |
| 25925-2 cl.22 E153G | 23 | >50 | 0.38 | >50 | 125.40 | 0.08 | 0.008 |
| CMT 19S531 | >50 | >50 | 0.68 | 13.22 | 39.00 | 0.06 | 0.01 |
| CMT 19S531 E153G | >50 | >50 | 0.53 | 8.63 | 40.00 | 0.03 | 0.008 |