FIG. 3.

Host and phage proteins during Syn5 infection. (a) SDS-PAGE (12%, Krypton stained) of supernatants (sup) and pellets (pel) of infected cells collected at different time points from 0.5 to 120 min after infection. M, protein standard (Bio-Rad, Precision plus); 8109, uninfected Synechococcus sp. strain WH8109 host cells collected 10 to 20 min before infection; Syn5, infectious particles used in the experiment and run as control for phage structural proteins. The gp (gene product) samples (right panel) contain purified recombinant phage proteins gp37, gp53, gp54, and gp58. Protein annotation: gp45, internal virion protein; gp46, tail fiber protein; gp41, tail tube B; gp44, internal protein; gp54, novel structural protein; gp37, portal; gp53, novel structural protein; coat, capsid protein; gp43, internal virion protein; gp40, tail tube A; gp55, gp57, and gp58, novel structural proteins. (b) Western blot of a gel run in parallel to the one shown in panel a, probed with a mixture of anti-Syn5 polyclonal antibodies and rabbit serum raised against the recombinant forms of gp53, gp54, and gp58.