FIG. 3.
DPV022 interacts with Bak. (A) S. cerevisiae cells were cotransformed with the indicated plasmids, and standardized concentrations were spotted in serial dilutions vertically down the plate. Galactose-containing media (on) induced the expression of Bak, whereas glucose-containing media (off) repressed the expression of Bak. Colony growth reflects resistance to Bak-induced growth suppression. (B and C) HEK 293T cells were cotransfected with pEGFP, pEGFP-FPV039, pEGFP-M11L, pEGFP-F1L, or pEGFP-DPV022 and pcDNA3-HA-Bak (B) or pEGFP, pEGFP-Bcl-xL, or pEGFP-DPV022 and pcDNA3-HA-Bak (C). zVAD.fmk was added after transfection to prevent caspase activation. Following lysis in 2% CHAPS buffer, lysates were immunoprecipitated (IP) with an anti-EGFP antibody and subjected to Western blotting (WB) with anti-EGFP or anti-HA antibodies to detect an interaction.