FIG. 9.

DPV022 inhibits the conformational activation of endogenous Bak and Bax during vaccinia virus infection. Wild-type Jurkat cells (a to e), Jurkat cells overexpressing Bcl-2 (f to i), and Jurkat cells devoid of Bak and Bax (j to m) were infected with VV-EGFP, VVΔF1L, or VVΔF1L-Flag-DPV022 at an MOI of 10 for 4 h (A) or 6 h (B) and then treated with 250 nM (A) or 2 μM (B) staurosporine (STS) for 2 h to induce apoptosis. Bak and Bax activations were quantified by flow cytometry using anti-Bak Ab-1 (A) and anti-Bax 6A7 (B) antibodies, respectively, or an isotype control antibody (NK1.1). Shaded histograms, untreated cells; open histograms, STS-treated cells. Data are representative of at least three independent experiments.