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. 2010 Dec 22;85(5):2276–2287. doi: 10.1128/JVI.01862-10

FIG. 4.

FIG. 4.

Sequence verification of VZV-specific transcripts in latently infected human trigeminal ganglia. Trigeminal ganglia from subjects containing ORF 11, 63, and 68 transcripts detected by multiplex RT-PCR analysis were sequence verified. The 3′-terminal sequences spanned positions 618 to 836 (ORF 63), positions 2189 to 2459 (ORF 11), and positions 1602 to 1871 (ORF 68) of the coding regions. All three transcripts contained the respective termination codon (boxed) and polyadenylation signal (underlined) and terminated in a poly(A) sequence (bolded). VZV ORF 41, 43, and 57 cDNA sequences mapped to nucleotide locations 445 to 616 (ORF 41), 249 to 363 (ORF 43), and 75 to 201 (ORF 57) and terminated within the reverse primers (italicized).