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. 2010 Dec 22;85(5):2189–2200. doi: 10.1128/JVI.01993-10

FIG. 3.

FIG. 3.

CD40L-bearing virions drive the expression of signal transduction regulators TRAF1 and A20. Tonsillar B cells were exposed for 8 or 24 h at 37°C to supernatants from 293T cells transfected with an empty control vector (mock), supernatants from 293T cells transfected with a vector coding for CD40L (mock/CD40L), NL4-3, NL4-3/CD40L, or anti-CD40. mRNA and protein levels of TRAF1 (A) and A20 (B) were assessed by qRT-PCR and Western blot assays, respectively. Results for mRNA amounts are expressed as fold changes above the mock control and are normalized on 18S expression. β-Actin was used as a loading control in the protein assay. The data shown are the means ± standard deviations for triplicate samples and are representative of two separate experiments performed with different donors. Asterisks denote statistically significant data (ANOVA test, Bonferronni-corrected P values; *, P < 0.05; **, P < 0.01).