FIG. 1.

Src knockdown inhibits ANDV-induced EC permeability. (A) Endothelial cells were transfected with Src or scrambled siRNA, and Src mRNAs were analyzed by qRT-PCR (69). mRNA levels were quantitated and standardized to GAPDH mRNA levels and presented as a percentage of controls. (B) Endothelial cells were plated on Transwell inserts and infected at an MOI of 0.5 in triplicate with ANDV. One day postinfection (p.i.), cells were transfected with control or Src-specific siRNAs using SureFECT siRNA transfection reagent. Three days p.i., VEGF (100 ng/ml) and FITC-dextran were added to medium in the upper chamber, and the presence of FITC-dextran in the lower chamber was quantitated at indicated times (26, 27, 31). The percent change in FITC-dextran over controls is presented as a measure of EC monolayer permeability (27).