FIG. 3.

VEGFR2-Src inhibitors block ANDV-induced permeability. Endothelial cells were plated on vitronectin-coated Transwell inserts and infected at an MOI of 0.5 in triplicate with ANDV. Three days postinfection, the permeability of ANDV- and mock-infected endothelial cell monolayers was determined as described for Fig. 1 (27) at indicated times in the presence or absence of the kinase inhibitors pazopanib (A), dasatinib (B), bosutinib (C), PP1 (D), and Src inhibitor 1 (E). The percent change in FITC-dextran over controls is presented as a measure of EC monolayer permeability (27). Data are derived from two independent experiments performed in triplicate with comparable results.