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. 2010 Dec 29;85(5):2060–2078. doi: 10.1128/JVI.02210-10

FIG. 3.

FIG. 3.

Pseudotyping NSCs facilitate CasBrE Env vector expression in host microglia and OPCs, but not mature oligodendrocytes. (A) Representative brain stem sections from mice transplanted with CasE+4070A and CasES+4070A NSCs immunostained for CasBrE Env (Env; green) and the microglial marker Iba-1 (Iba-1; red) and then merged to show colocalization. Env-positive microglia were readily detected in areas with or without spongiform pathology, as indicated by arrowheads; however, there were also areas where Env expression was observed with limited or no colocalization to microglia (far right images). Images were obtained by using epifluorescence optics. Bar, 40 μm. (B) Confocal double-immunofluorescence staining of CasE, CasES, 4070A, CasE+4070A, and CasES+4070A NSC-transplanted brains with antibodies specific for the mature oligodendrocyte markers CNPase (red; top row) and carbonic anhydrase II (red; second row); the oligodendrocyte progenitor (OPC) markers NG2 (red; third row) and Olig2 (red; bottom row); and either CasBrE Env (Env; green) in CasE, CasES, CasE+4070A, and CasES+4070A NSC transplants or Gag (Gag; green) in 4070A NSC transplants. Arrowheads (white) indicate examples of cells showing Env or Gag colocalization with an oligodendrocyte cell type-specific marker. Bar, 40 μm.