TABLE 4.
Cell type expression of retroviral proteins in brain stems transplanted with packaging NSCs
| CNS cell type | Marker | No. of cells positive for glial cell marker/total no. of cells (%) in transplantsa |
||||
|---|---|---|---|---|---|---|
| gpgpt (MAb R187)b | CasE-gpgpt |
FE-gpgpt |
||||
| MAb 697c | MAb R187 | FEd | MAb R187 | |||
| Oligodendrocyte | CAII | 4/325 (1.7) | 1/438 (0.2) | 2/278 (0.7) | 6/300 (2) | 5/376 (1.3) |
| CNPase | 0/262 | 0/220 | 0/25 | 1/371 (0.3) | 1/241 (0.4) | |
| Astrocyte | GFAP | 0/273 | 0/308 | 0/246 | 0/40 | 0/194 |
| Olig2+ cell | Olig2 | 14/234 (5.6) | 1/358 (0.3) | 5/92 (5.4) | 7/321 (2.2) | 10/233 (4.5) |
| NG2+ cell | NG2 | 60/176 (34.1) | 49/166 (29.5) | 47/159 (29.6) | 68/233 (29.2) | 62/363 (17.1) |
a
The numbers provided indicate the number of cells positive for the glial cell marker (numerator) out of the total number of Env or Gag positive cells observed in the brain stem sections surveyed (denominator). Colocalization was determined by z-stack analysis of confocal images.
b
MAb R187 (8) was used to detect Gag protein expression.
c
MAb 697 (36) was used to detect CasBrE Env protein expression.
d
Goat anti-Friend Env antiserum (FE) was used to detect Friend Env expression.