FIG. 1.

The tsB7 UL36 gene contains four amino acid substitutions. (a) The UL36 genes from tsB7, parental strain HFEM, and wt strain 17 were sequenced. Positions of amino acid substitutions (numbered with reference to the HFEM sequence [see Materials and Methods]) are indicated together with the residues at these positions in tsB7 or the other strains. Features of VP1-2 illustrated in the diagram include the ubiquitin-specific protease (USP), nuclear localization signal (NLS), UL37-binding domain (UL37BD), and conserved C-terminal UL25-binding domain (UL25BD). The positions of fragments used for marker rescue of tsB7 encompassed by SalI or XhoI sites are also indicated (3). (b) Sequence homology around the tsB7 substitutions in the VP1-2 proteins of alphaherpesvirus subfamily members. Alignments were made using ClustalX alignment software and illustrated using Genedoc. Residues with complete identity allowing for close chemical similarity are indicated in white text with a black background, over 85% by white text on dark gray, and over 50% by black text on light gray.