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Sequence-independent binding of RNA by recombinant VPgs. The smallest amount of VPgWT (90 ng) bound part of the labeled RNA, whereas 90 ng VPgΔ38-44 did not show any retardation of RNA. A gradual increase of the free RNA was seen from VPgNTP1 to VPgNTP3 when 300 ng of the proteins was added into the assay.
