TABLE 5.

Proteins predicted to be involved in virus transmission in the accessory salivary glands of Schizaphis graminum aphids based on the coupling of genetic and DIGE data

a ESI-MS/MS on a 4000QTrap instrument was used for protein identification.

b Multiple proteins were identified in the spot.

c ESI-MS/MS on a Synapt HDMS instrument was used for protein identification.

d MALDI-MS/MS was used for protein identification.

e Interpro Scan SignalP hidden Markov model.

f Average normalized volume appears upregulated, but difference is not statistically significant.

g Mean separations of log-normalized volumes are shown via shading and underlining, with shading indicating higher expression levels on a spot-by-spot basis (ANOVA P value, <0.05). Sample spot volumes were normalized using a background-adjusted abundance ratio to the Cy2 internal standard and were adjusted by a gain factor calculated for each sample.

h The fold change value reported is the maximum change between the mean normalized volumes between genotypes.