Skip to main content
. 2010 Dec 15;85(5):2351–2363. doi: 10.1128/JVI.01621-10

FIG. 8.

FIG. 8.

ILTV-encoded UL49.5 does not affect TAP function. (A) Surface expression of MHC-I (LMH and MJS) and MHC-II (MJS) molecules was assessed by flow cytometry on untransduced cells (graph 2) and ILTV UL49.5-expressing cells (graph 3) with the indicated antibodies. Graph 1, secondary antibody only. (B) Transport activity of TAP was analyzed in LMH and MJS expressing ILTV UL49.5. Peptide transport was evaluated in the presence of ATP (▪) or EDTA (□). (C) The steady-state levels of UL49.5 (MJS and LMH) and TAP1 (MJS) were determined by using SDS-PAGE and immunoblotting (IB) with the indicated antibodies. The β-actin signal was used as a loading control.