FIG. 5.

The rad21-45 mutation affects the distribution of heterochromatin and euchromatin marks, both of which are present at the Chr1-R subtelomeric region. Cells were cultured under cycling conditions at 25°C. (A) RT-QPCR analysis of gene expression in cycling rad21-45 mutant cells. Data are expressed as the fold change versus the wild type (wt). Error bars represent the standard errors of the means from two independent biological replicates. (B and C) H3K9me (B) and H3K4me (C) features of heterochromatin and euchromatin, respectively, were analyzed by ChIP at the Chr1-R subtelomeric region. Error bars represent the standard errors of the means from duplicate samples.