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. 2010 Dec 28;31(5):924–934. doi: 10.1128/MCB.00576-10

FIG. 1.

FIG. 1.

Requirement of TBL1 for NF-κB target gene expression. (A) Knockdown of TBL1 in M4e cells by shRNA. Western blotting was performed using anti-TBL1, anti-p65, anti-TBLR1, and anti-α-tubulin antibodies. (B) Knockdown of TBL1 inhibited TNF-α-stimulated NF-κB target gene expression in M4e cells. M4e/Scrsh and M4e/TBL1sh cells were stimulated with TNF-α for the indicated times. The expression levels of IκBα and c-IAP2 were determined by quantitative RT-PCR. Values are means ± standard deviations of triplicate samples from a representative experiment. (C) Knockdown of TBL1 in M4e cells by siRNA. M4e cells were transiently transfected with scramble siRNA (Scrsi) or TBL1 siRNA (TBL1si). TBL1 expression was determined by the real-time RT-PCR. (D) Knockdown of TBL1 by siRNA inhibited TNF-α-induced NF-κB target gene expression in M4e cells. The expression levels of IκBα and c-IAP2 were determined by quantitative RT-PCR. (E) Knockdown of TBL1 in MDA-MB-231 cells. Western blotting was performed using anti-TBL1, anti-p65, anti-TBLR1, and anti-α-tubulin antibodies. (F) TBL1 knockdown inhibited NF-κB target gene expression in MDA-MB-231 cells. MDA/Scrsh and MDA/TBL1sh cells were stimulated with TNF-α for the indicated times. The expression levels of IκBα and c-IAP2 were determined by quantitative RT-PCR. Values are means ± standard deviations of triplicate samples from a representative experiment.