FIG. 7.

Only the STB-proximal segment can retain association with the Cse4 directed antibody following pulldown and restriction enzyme digestion of a reporter plasmid. The four tandem copies of BamHI and BglII recognition sites that cordon off STB-proximal in the reporter plasmid are diagrammed schematically. Plasmid pulldown and Southern blot analysis of the BamHI- plus BglII-digested DNA were conducted as described in the legend to Fig. 3. BI + BII (above the lane showing results for the digestion of naked DNA) refer to BamHI and BglII, respectively.