Generation of bronchoalveolar epithelium-specific Akt1 TG mice. (A) Schematic illustration of binary TG system. (B) Western blot analysis of the whole-lung lysate from control and DTG mice at P0. (C) Respiratory rate at P0. DOX-treated SP-C-rtTA TG mice do not show respiratory distress. *, P < 0.05 versus DOX-treated wild-type, Tet-myrAkt1 TG mice, and SP-C-rtTA TG mice (n = 7, 5, 6, and 6 mice, respectively, from 3 dams). All animals were treated with DOX in the drinking water. (D) HE staining of the lung sections. Scale bar, 50 μm.