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. 2010 Dec 20;31(5):1066–1075. doi: 10.1128/MCB.00883-10

FIG. 1.

FIG. 1.

Association of ARHGAP21 and β-arrestin 1 by yeast 2-hybrid and peptide array analyses. (Top) Schematic diagram showing the functionally relevant areas of ARHGAP21. Overlapping sequences within ARHGAP21 that were shown to interact with β-arrestin 1 by yeast 2-hybrid analysis (shaded rectangle) and peptide array (filled rectangle) are indicated. (Bottom) Immobilized peptide spots of overlapping 25-mer peptides, each shifted along by 5 amino acids in the entire sequence of ARHGAP21, probed for interaction with either β-arrestin 1-GST, β-arrestin 2-GST, or GST alone. Positive interactions were detected in amino acid sequences spanning positions 1321 to 1360 of ARHGAP21.