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Alanine scanning arrays of the β-arrestin 1 binding site on ARHGAP21. The alanine substitution array for the P1321-to-P1360 peptide (Cont) (top leftmost spot) from ARHGAP21was probed with β-arrestin 1-GST. A1343 was replaced with an aspartate residue. Multiple substitutions were also undertaken as indicated (bottom).
