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. 2010 Dec 20;31(5):1066–1075. doi: 10.1128/MCB.00883-10

FIG. 3.

FIG. 3.

β-Arrestin 1 binds to the GAP domain of ARHGAP21. (Top) Purified GST fusions with the indicated regions of the sequence of ARHGAP21 (amino acids 885 to 1346) and GST alone were mixed with purified MBP fusions of β-arrestin 1. (Bottom) Inputs of purified β-arrestin 1-MBP and ARHGAP21-GST constructs (left) and pulled down GST fusion proteins (right) were blotted for the presence of β-arrestin 1-MBP. Asterisks indicate bands that correspond either to an ARHGAP21-GST construct (constructs 1 to 7) or to GST alone (first lane).