FIG. 1.

HPV-18 E6 protein binds to EDD in vitro and in vivo. (A) Radiolabeled in vitro translated EDD was incubated with GST, GST-18E6, GST-16E6, GST-11E6, and GST-18E6*. Bound proteins were assessed by autoradiography, and the input GST fusion proteins were visualized with Coomassie staining (lower panel). Input EDD (10%) is shown. (B) 293 cells were transfected with HA-tagged HPV-18 E6 (HA-18E6), EDD1, or HA-tagged MAGI-2, as indicated. After 24 h cells were incubated for 3 h with MG132 before being harvested, and cell extracts were immunoprecipitated with anti-HA antibody. Coprecipitating EDD was detected by Western blotting with anti-EDD antibody. The three lower panels show the protein inputs of EDD, MAGI-2, and HPV-18 E6.