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. 2011 Jan 19;85(7):3356–3366. doi: 10.1128/JVI.02105-10

FIG. 3.

FIG. 3.

ICP0 mediates polyubiquitylation of E2FBP1 through its RING/HUL-2 domain. (A) Schematic representation of ICP0 and its deletion mutants. (B) ICP0 lacking the RING/HUL-2 domain fails to induce E2FBP1 polyubiquitylation. Expression plasmids were transformed into HEK293FT cells at the ratios indicated at the top of the figure. Cell lysates were prepared after 20 h of transformation and subjected to immunoblotting (lanes 1 to 4) (top, ICP0; bottom, HA-E2FBP1) and Ni-NTA pulldown followed by immunoblotting (lanes 5 to 8) (HA-E2FBP1). Positions for nonubiquitylated and ubiquitylated HA-E2FBP1 and ICP0 mutant proteins are shown to the left of the figure.