TABLE 1.
Primers and combined hydrolysis probes designed for quantitative PCRa
| Target | GenBank accession no. | Primer direction | Sequence | Probe |
|---|---|---|---|---|
| E2FBP1 | NM_005224 | Forward | GCACTCCAGCAGAACTTCCT | UPL 44 |
| Reverse | AGAGTCCTGGCGGCTTTC | GCTGCCCA | ||
| ICP0 | X04614 | Forward | AGCCCCGTCTCGAACAGT | UPL 56 |
| Reverse | ACCACCATGACGACGACTC | TGCTGTCC | ||
| IRES | M81861 | Forward | TGGCTCTCCTCAAGCGTATT | UPL 41 |
| Reverse | CCCATACAATGGGGTACCTTC | GGCTGAAG | ||
| 18S rRNA | X03205 | Forward | CGATTGGATGGTTTAGTGAGG | UPL 81 |
| Reverse | AGTTCGACCGTCTTCTCAGC | GGCCCTGG | ||
| RPPH1 | NR_002312 | Forward | CCGGAGCTTGGAACAGACT | UPL 30 |
| Reverse | GTAGTCTGAATTGGGTTATGAGGTC | GGCTGAGG |
a
UPL, universal probe library probe provided by Roche Applied Science; IRES, cap-independent translation enhancer sequence from encephalomyocarditis virus polyprotein gene (nucleotides 335 to 834) subcloned into pEF-x-IRESP; RPPH1, RNase P RNA component H1.