FIG. 7.

Knockdown of genes required for repression of cryptic promoters reactivates latent HIV. (A and B) J-Lat E27 cells were infected with control, ASF1a, HIRA, or SUPT16H shRNA expression lentiviruses and selected in puromycin as described for Fig. 5A, and HIV-GFP expression was measured by RT-qPCR with oligonucleotides 8 and 9. GAPDH expression was used for normalization. The fold change of HIV induction in cellular factor-depleted cells compared to that in control cells is indicated. Depletion of these factors was tested by RT-qPCR with specific primers and is shown in panel A. Values represent the mean and range of a representative experiment performed in duplicate. (C) J-Lat E27 and J-Lat pool cells were infected with control or ASF1a shRNA as described for panels A and B, and HIV-GFP expression was measured by fluorescence-activated cell sorting. Data are expressed as percentages of GFP-positive cells (%GFP).