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. 2010 Dec 29;85(7):3546–3556. doi: 10.1128/JVI.02045-10

FIG. 7.

FIG. 7.

Full-length as well as dominant negative ARRDC1 colocalizes with aberrant endosomes induced by dominant negative VPS4. HeLa cells were transiently transfected with expression constructs encoding mCherry-ARRDC1 or mCherry-ARRDC1NC and dominant negative YFP-VPS4 (YFP-VPS4dn). Cells were fixed, and nuclei were stained using Hoechst stain and analyzed by confocal microscopy for colocalization of ARRDC1 proteins with aberrant endosomes induced by the expression of dominant negative VPS4. Pictures shown are representative of three independent experiments. Colocalization of ARRDC1 constructs with dominant negative VPS4 was quantified by calculating the Pearson's correlation coefficient using Image J public domain image processing software (plug-in). The following values were obtained: mCherry-ARRDC1/YFP, 0.16 ± 0.04 (n = 3); mCherry-ARRDC1/YFP-VPS4dn, 0.66 ± 0.10 (n = 7); mCherry-ARRDC1NC/YFP, −0.24 ± 0.15 (n = 7); mCherry-ARRDC1NC/YFP-VPS4dn, 0.76 ± 0.15 (n = 5). Data are represented as average value ± standard deviation. n, number of analyzed cells.