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. 2011 Jan 19;85(7):3270–3282. doi: 10.1128/JVI.02243-10

FIG. 5.

FIG. 5.

M062 is essential for MYXV infection in many diverse human cells. (A) Multiple-step growth curves of MYXV in U87 glioma cells. U87 cells were infected at an MOI of 0.1 with either vMyx-gfp or vMyxM062-KO. At given time points (12, 24, 48, and 72 h p.i. for both viruses and up to 96 h p.i. for vMyxM062-KO), total cell lysates were harvested for titration. Error bars represent ± the SD generated from titration in triplicate, and the results of a representative growth curve of two independent experiments are shown. (B) One-step growth curves of MYXV infection in U87 cells. U87 cells were infected at an MOI of 3 with vMyx-gfp or vMyxM062-KO. Cell lysates were harvested at 2, 4, 12, 24, and 57 h p.i. for titration, and error bars represent ± the SD generated from titration in triplicate. The results of a representative growth curve from two independent experiments are shown. (C) Multiple-step growth curves of MYXV in Huh7 cells. Experiments were carried out as described for panel A. (D) One-step growth curves of MYXV in Huh7. Experiments were carried out as described for panel B. (E) Multiple-step growth curves in HepG2 cells. Experiments were carried out as described for panel A. (F) One-step growth curves in HepG2 cells. Experiments were carried out as described for panel B.