TABLE 1.
Role of M062 during in vivo pathogenesis by MYXV in European rabbits
| Infection group | Observation(s)a |
|
|---|---|---|
| vMyx-Lau (wt) or vMyxM062-Rev | vMyxM062-KO | |
| Initial infection (intradermal inoculation of 1,000 FFU) | At 2 to 3 dpi, there was a light pink area at the infection site; by 5 dpi, secondary lesions were visible; at 7 dpi, there were extensive primary and secondary lesions, animals' attitude was dull; at 10 to 11 dpi, there was severe respiratory stress and nasal discharge with reduced input/output and no or low activity (endpoint) | No signs of primary or secondary lesions were observed, and all animals remained healthy throughout the time of observation |
| Surviving animals from the initial infection were challenged intradermally with 1,000 FFU of vMyx-Lau (wt) | Challenge with vMyx-Lau took place at 24 dpi after the initial infection with vMyxM062KO. A full-blown infection occurred at up to 5 dpc, and there was no obvious protection in reducing primary or secondary lesion size or numbers. At 7 dpc, primary and secondary lesions started healing. At 10 dpc, three animals showed respiratory stress, while the other two animals fully recovered without respiratory complications | |
a
dpi, days postinfection; dpc, days postchallenge.