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. 2011 Jan 26;85(7):3683–3689. doi: 10.1128/JVI.02112-10

FIG. 4.

FIG. 4.

(A) Numbers of unique Tat28-35SL8-specific CD8+ T-cell clonotypes in macaques following vaccination with SIVmac239Δnef or DNA prime-Ad5 boost with Tat-encoding vectors, estimated for a standard sample size of 70 TCRβ sequences across all samples (20). (B to E) Frequencies of Tat28-35SL8-specific CD8+ T-cell clonotypes in macaques following vaccination with SIVmac239Δnef or DNA prime-Ad5 boost with Tat-encoding vectors that feature particular CDR3 amino acid (a.a.) motif characteristics (B), CDR3 lengths (C), TRBJ gene usage (D), and TRBV gene usage (E). (F to J) Comparisons of TCR repertoire parameters between macaques at week 2 after SIVmac239Δnef vaccination, week 8 after SIVmac239Δnef vaccination, and week 2 after Ad5 boost; number of unique Tat28-35SL8-specific CD8+ T-cell clonotypes (F), CDR3-6R motif frequency (G), frequency of CDR3s with lengths of 13 amino acids (H), frequency of TRBJ1-5 gene usage (I), and frequency of TRBV27 gene usage (J). (K) Relationship between the frequency of Tat28-35SL8-specific CD8+ T-cell clonotypes with the CDR3-6R motif at week 8 after SIVmac239Δnef vaccination and the frequency of wild-type (WT) Tat28-35SL8 sequences observed at week 3 after SIVmac239Δnef vaccination.