FIG. 4.

Analysis of differentially expressed genes between two libraries. (A) Summary of the numbers of differentially expressed genes in the Tomato yellow leaf curl China virus viruliferous whiteflies. “FDR < 0.001 and the absolute value of log₂ ratio ≥ 1” were used as the threshold to judge the significance of gene expression difference. (B) Fold change distribution of differentially expressed genes. (C) Correlation analysis of two libraries. The Pearson correlation coefficient for two libraries is shown in the upper left corner of the plot. (D) Comparison of DGE data and qPCR results. “Concordant up” means that genes in the viruliferous whiteflies were upregulated for both DGE and qPCR analyses. “Concordant down” means that genes in the viruliferous whiteflies were downregulated for both DGE and qPCR analyses. “Different” means that, for DGE and qPCR analyses, the direction of change of gene expression in the viruliferous whiteflies was contrary.