FIG. 3.
Fusion proteins inhibit lentiviral infection. (A) Western blotting of MT4 cell lysates with antibody to GFP. Tubulin was immunoblotted as a loading control. (B) HIV-1luc challenge of stable MT4 cells. WT/D366N pairs are indicated. Values were normalized to cell number or protein. Error bars are between duplicates in each experiment. All challenge experiments were performed at least twice with similar results. In panels B to D, cell lysates were collected 5 to 7 days after infection. (C) FIV-luc infection. (D) MLV-luc infection. (E) Alu-PCR assessment of integration. Values are normalized to GAPDH standard. (F) 2-LTR circles, analyzed at 22 h posttransduction with HIV-1luc. Values are normalized to MtDNA as a loading control.