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. 2011 Jan 26;85(7):3570–3583. doi: 10.1128/JVI.01295-10

FIG. 9.

FIG. 9.

Inhibition of HIV-1 infection by PK-GFP-IBD. (A) MT4 cell lines stably expressing GFP-IBD, GFP-IBDD366N, PK-GFP-IBD, and PK-GFP-IBDD366N,5E were challenged with HIV-1luc. (B) Alu-PCR based integration assay of HIV-1luc in MT4 PK-GFP-IBD cells. (C) Western blot of subcellular fractions of MT4 cells expressing either GFP-IBD+LEDGFshRNA or PK-GFP-IBD+LEDGFshRNA. LEDGF is markedly decreased in both cell lines. See the Fig. 5A legend for assay details. (D) MT4 cells stably expressing PK-GFP-IBD, a LEDGF-targeted shRNA and GFP-IBD, or a LEDGF-targeted shRNA and PK-GFP-IBD were challenged with HIV-1luc. (E) LAI luciferase challenge of indicated cell lines. Luciferase was analyzed 4 days after infection. (F) Time course of HIV-1luc 2-LTR circle formation in the indicated cell lines. (G) Mean nuclear fluorescence intensity versus DI activity. The MFI was obtained for 10 control MT4 cells, and 20 each of GFP-IBD, GFP-IBDLOW, and PK-GFP-IBD cells (see Fig. S1A to C in the supplemental material). The legend at the bottom indicates whether the cell line produces antiviral dominant interference versus MT4 control cells.