TABLE 1.
PCR primers
| Construct | Junction | Primer sequence (5′-3′) |
|---|---|---|
| GFP-199-432 | VKQPCP | atatccgcggtaaaacagccctgtccttcagag |
| ttctcgaggtacctatccttcaccaaccaagaacatgttc | ||
| GFP-266-432 | KTGVTST | atatccgcggggaaaacaggggttacttcaacc |
| ttctcgaggtacctatccttcaccaaccaagaacatgttc | ||
| GFP-325-432 | EQQNKDEGKK | atatccgcggagcagcagaataaagatgaagg |
| ttctcgaggtacctatccttcaccaaccaagaacatgttc | ||
| GFP-199-530 | VKQPCP | atatccgcggtaaaacagccctgtccttcagag |
| atatggtaccctagttatctagtgtagaatc | ||
| GFP-266-530 | KTGVTST | atatccgcggggaaaacaggggttacttcaacc |
| atatggtaccctagttatctagtgtagaatc | ||
| GFP-325-530 | EQQNKDEGKK | atatccgcggagcagcagaataaagatgaagg |
| atatggtaccctagttatctagtgtagaatc | ||
| NETC-GFP-IBD | atatggatccaccatgtcaggagccggaggagtcggatctgcagatggtgagcaagggc | |
| atatctgcagaggggattccaacatggaaattgtcgacctatccttcaccaaccaa | ||
| NETC-GFP-IBDD366N | atatggatccaccatgtcaggagccggaggagtcggatctgcagatggtgagcaagggc | |
| atatctgcagaggggattccaacatggaaattgtcgacctatccttcaccaaccaa | ||
| PK-GFP-IBD | atatctcgaggccgccaccatgctgcacgctgccatggcagacacctttctg | |
| atatggatcccctggcacgggcaccacccgc | ||
| PK-GFP-IBDD366N,5E | atatggatccaccggtcgccaccatggtgagcaagg | |
| cttttccatgattacctgactctctttctcctcccgtatttcttccagtgtagtaatc | ||
| gattactacactggaagaaatacgggaggagaaagagagtcaggtaatcatggaaaag | ||
| atatactagtgctagagattttccacactgac |