TABLE 2.
Cell line | shRNA | Protein(s) expressed |
---|---|---|
Adherent cell lines | ||
293T | None | None |
L1340 | p75 1340 | None |
LH4 | p75 1340 | HIV-1 IN |
CD4+ T cell lines | ||
SupT1 | None | None |
TC3 | Control | mCherry |
TL3 | p75 1340 | mCherry |
SupT1 GFP-IBD | None | GFP-IBD (345-432) |
SupT1 GFP-IBDD366N | None | GFP-IBDD366N (345-432) |
SupT1 GFP-IBD + p75shRNA | p75 1340 | GFP-IBD (345-432) |
SupT1 GFP-IBD + p75shRNA + p75syn7 | p75 1340 | GFP-IBD (345-432) + LEDGF |
SupT1 GFP-199-530 | None | GFP-199-530 |
SupT1 GFP-199-530D366N | None | GFP-199-530D366N |
MT4 | None | None |
MT4 Scr GFP | Control | GFP |
MT4 GFP-IBD | None | GFP-IBD (345-432) |
MT4 IBD-GFP | None | IBD-GFP (345-432) |
MT4 GFP-IBDD366N | None | GFP-IBDD366N (345-432) |
MT4 GFP-IBD + p75shRNA | p75 1340 | GFP-IBD (345-432) |
MT4 GFP-IBDD366N + p75shRNA | p75 1340 | GFP-IBDD366N (345-432) |
MT4 GFP-199-530 | None | GFP-199-530 |
MT4 GFP-199-530D366N | None | GFP-199-530D366N |
MT4 199-432 | None | GFP-199-432 |
MT4 266-432 | None | GFP-266-432 |
MT4 266-432D366N | None | GFP-266-432D366N |
MT4 325-432 | None | GFP-325-432 |
MT4 325-432D366N | None | GFP-325-432D366N |
MT4 266-530 | None | GFP-266-530 |
MT4 325-530 | None | GFP-325-530 |
MT4 NETC-GFP-IBD | None | NETC-GFP-IBD |
MT4 NETC-GFP-IBDD366N | None | NETC-GFP-IBDD366N |
MT4 PK-GFP-IBD | None | PK-GFP-IBD |
MT4 PK-GFP-IBDD366N,5E | None | PK-GFP-IBDD366N,5E |
MT4 PK-GFP-IBD + p75shRNA | p75 1340 | PK-GFP-IBD |