FIG. 4.

MTKDEL is retained in the ER, whereas MTGGDV and MTSec61β are not. Immunofluorescence analysis was performed on cells transiently expressing the mutants shown in Fig. 3. Cells were transfected with each of the mutants, and, after 48 h, fixed, and stained with PAb762 anti-MT monoclonal antibody. Images were then taken at high magnification. The mutant expressed is shown either above or below each micrograph.