FIG. 6.

Association with PP2A is required for MT migration to the plasma membrane. Immunofluorescence analysis was performed on cells expressing various mutants of MT using anti-MT monoclonal antibodies that react with different regions of the molecule. Cells were plated onto coverslips, fixed, and then reacted with individual antibodies. Images were then taken at high magnification. Above each panel, the MT mutant expressed is noted, followed by the monoclonal antibody used to detect the MT. Mutants DC1 and NS2 fail to bind PP2A and, consequently, most of the other signaling proteins; mutant ΔJ-DC1 fails to bind Hsc70 and PP2A. Antibody PAb762 reacts with the N terminus of MT, PAb754 reacts with the C terminus, and PAb702 reacts with an N-terminal site that is required for binding PP2A.