FIG. 5.

Analysis of analogs of compound 7. (A) Summary of preliminary SAR results for benzodiazepine analogs of HIV/EBOV-GP compound 7. (B) Comparison of antiviral activity and cell toxicity of compound 12, as described in Materials and Methods. The blue diamonds represent anti-GFP-ZEBOV activity, while the red squares represent cytotoxicity. (C) Effect of compound 12 on the infectivities of N69A, L70A, L184A, I185A, L186A, and K190A/K191A HIV/EBOV-GP mutants. 293 T cells were infected with HIV/EBOV-GP pseudotype virus in the presence of increasing concentrations of compound 12. Inhibition of HIV/EBOV-GP infection was detected as a reduced luciferase signal. Error bars indicate standard deviations.