FIG. 5.

Neutralization of species A and B and “swapped” chimeras by rabbit antibody LMF87 raised against the HVR1 peptide of species A. (A) Huh7.5 cells were transfected with RNA transcripts from species A (black bars) or B (white bars), viruses in the medium at day 4 posttransfection were mixed with dilutions of LMF87 or control serum, and the number of infectious viruses was determined in a focus-forming assay. % Neutralization = 1 − (FFU in LMF87 sample/FFU in control sample) × 100. (B) HVR1 regions of species A (black) and species B (white) were swapped by PCR mutagenesis, and neutralization by LMF87 was determined as described for panel A. Error bars show standard deviations.