FIG. 6.

Effects of backbone mutations on virus outgrowth following neutralization by LMF87 antibody raised against the HVR1 peptide of species A. (A and B) Diagrams of species A (black) and B (white) pairs tested. (C and D) Respective percentage of neutralization by LMF87. Error bars show standard deviations. (E and F) Mixtures (50:50) of species A and B as diagramed in panels A and B were incubated with control serum or with dilutions of LMF87 anti-HVR1 prior to inoculation onto Huh7.5 cells. After 42 days of culture, the HVR1 sequences of viruses in the medium were sequenced to determine the species distribution. Species A (black); species B (white); new mutation in HVR1 (hatched).